Figure 3.

BORFF3–4 encodes a functional β1,6GnT forming core 2 and I structures. CHO-Py⋅leu cells were transfected with pcDNA3.1 (A and B), pcDNA3.1-BORFF3–4 (C and D), or pcDNA3.1-C2GnT-M (E and F) by using FuGENE 6 transfection reagent (Roche). Twenty-four hours after transfection, the cells were treated for indirect immunofluorescent staining of fixed samples. mAb T305 reacting with core 2 oligosaccharides in leukosialin (A, C, and E) and α-I serum (B, D, and F) were used as primary Abs and were revealed by Alexa-GAM and PE-GAH secondary Abs, respectively. The percentage of positive cells estimated by examination of 600 randomly selected cells is presented in each panel. (Bar, 10 μm.)