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. 2001 Dec;159(6):2303–2308. doi: 10.1016/S0002-9440(10)63080-5

Figure 2.

Figure 2.

Association of CD2AP and nephrin in podocyte cell line. A: Insolubility of nephrin and CD2AP from purified glomeruli. Glomeruli were purified from mouse kidneys by sieving. Lysates were prepared with digitonin based lysis buffer. A portion of the soluble material (S) and a portion of the insoluble material (I) was analyzed by SDS-PAGE and immunoblotting with antibodies to CD2AP and nephrin. B: Lysate were prepared from a podocyte cell line. Equal amounts of the clarified lysate were incubated with either pre-immune rabbit serum (C) or affinity purified CD2AP antibody (CD2AP). Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting with anti-nephrin. Either whole cell lysate (-) or anti-nephrin immunoprecipitate (Nep) were loaded as positive controls.