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. Author manuscript; available in PMC: 2007 Apr 10.
Published in final edited form as: Steroids. 2006 Dec 14;72(2):188–201. doi: 10.1016/j.steroids.2006.11.009

Figure 5.

Figure 5

Phospho-mimic S294D PR-B exhibits decreased stability and increased transcriptional activity. A. Western blots showing ligand-induced downregulation of wt and S294D PR-B. HeLa cells were transiently transfected with either wt or S294D PR-B and treated with or without R5020 for 1–24 hours. Western blots for PR were performed using PR specific antibodies. B. T47D cells stably expressing either wt or S294D PR-B were treated for either 8 or 24 with R5020 and Western blots were performed using PR specific antibodies. C. HeLa cells were transiently transfected with wt, S294D or S294A PR-B, PRE-luciferase, and pRL-TK (Renilla). Cells were serum-starved for 24 hrs and then treated for 24 hours with R5020, EGF, or both agents (added simultaneously) and luciferase units in whole cell lysates were normalized to the signal from renilla as a transfection control.