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Integrity of 35S-labeled in vitro transcripts, as ascertained by PAGE. Lane 1, 3′-BC1 segment, preinjection aliquot; lane 2, 3′-BC1 segment, postinjection aliquot; lane 3, full-length BC1 RNA, preinjection aliquot; 8% acrylamide, Tris-borate/EDTA buffer. Arrow a indicates the position of full-length BC1 RNA (152 nt); arrow b indicates the position of the 3′-BC1 segment (60 nt).
