Figure 6.

Ocular VEGF levels in three transgenic mouse models. Mice from the V6 line of rho/VEGF transgenic mice (V6) were sacrificed at postnatal day 21 and eye homogenates were prepared. At postnatal day 21 double-transgenic rho/rtTA-TRE/VEGF (Opsin) and IRBP/rtTA-TRE/VEGF mice (IRBP) were treated with 0.5 mg/g body weight of doxycycline by subcutaneous injection for 3 days and then sacrificed. Eye homogenates were prepared, protein levels were measured, and then VEGF levels were measured by enzyme-linked immunosorbent assay as described in Materials and Methods. Bars represent the mean (± SEM) level of VEGF per μg total protein calculated from eye homogenates in each group.