Expression of S100A4 mRNA by normal and transfected cell lines. Ten μg of total RNA were subjected to electrophoresis in gels containing formaldehyde, blotted onto nitrocellulose filters, and hybridized with radiolabeled cDNAs corresponding to rat S100A4 (top) or rat glyceraldehyde-3-phosphate dehydrogenase (bottom). The different lanes were hybridized with RNA from MYU-3L parental cells, S100A4-transfected cells (MYU-C2, C7, C6, and C14), or control (CON) MYU-3L cells transfected with pSV2neo (MYU-plasmid cells).