Expression of integrins on monocytes or monocyte-derived macrophages. Integrin expression was analyzed on monocytes after initial adhesion after 1.5 hours (A, B, and C) or on day 3 of culture (D, E, and F). Permeabilized cells were stained with mouse monoclonal anti-integrin detecting antibodies (Table 1) ▶ and anti-mouse IgG conjugated to Cy-5. A and D, β1 integrin; B and E, β2 integrin; and C and F, β3 integrin. Representative images are of single 2-μm slices at the cell/substrate interface. Scale bars, 50 μm.