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Inhibition of HGF by hypoxia in activated HSCs is not mediated by cAMP or TGF-β1. A: Quantification of cAMP concentration by radioimmunoassay in HSCs under normoxia (open bars) and hypoxia (filled bars) for 24 hours (means ± SEM, n = 3). B: The expression of precursor (54 kd) and mature (13 kd) forms of TGF-β1 was studied by Western blot in HSCs maintained under normoxia and exposed to hypoxia for 24 hours.
