Table 1.
Effect of in Vivo Chemokine Depletion on Cell Composition of Dispersed Type-2 Granulomas in Wild-Type CBA Mice
| Treatment | Lymphocytes | Macrophages | Eosinophils | Neutrophils |
|---|---|---|---|---|
| Control IgG | 45.0 ± 4.2 | 30.0 ± 1.4 | 21.0 ± 4.2 | 3.5 ± 0.7 |
| Anti-ETX | 41.0 ± 7.9 | 36.3 ± 2.5 | 19.0 ± 5.2 | 3.6 ± 1.5 |
| Anti-MCP-3 | 54.5 ± 3.5 | 29.0 ± 1.4 | 12.0 ± 4.2* | 3.5 ± 2.1 |
| Anti-ETX+ anti-MCP-3 | 48.5 ± 2.1 | 34.5 ± 4.9 | 12.0 ± 1.4* | 4.5 ± 0.7 |
Values are mean ± SD of a representative experiment of three separate experiments with similar results; six to eight mice per group. Mice with type-2 pulmonary granuloma formation were treated with 10 mg of anti-chemokine antibodies or control IgG. On day 4, pulmonary granulomas were isolated, dispersed, and duplicate cytospin preparations were subjected to a 200-cell differential.
*P < 0.05 compared with control IgG group.