Table 2.
Effect of Eotaxin Knockout and MCP-3 Depletion on Cell Composition of Dispersed Type-2 Granulomas
| Mouse strain | Treatment | Lymphocytes | Macrophages | Eosinophils | Neutrophils |
|---|---|---|---|---|---|
| Eotaxin +/+ | None | 53.0 ± 5.6 | 23.0 ± 2.1 | 20.5 ± 0.8 | 3.5 ± 2.1 |
| Eotaxin−/− | None | 49.5 ± 0.7 | 22.5 ± 0.7 | 27.0 ± 1.4 | 1.5 ± 0.8 |
| Eotaxin−/− | IgG | 43.0 ± 4.0 | 27.0 ± 1.4 | 25.0 ± 2.0 | 6.0 ± 2.1 |
| Eotaxin−/− | Anti-MCP-3 | 53.0 ± 2.1 | 24.0 ± 1.4 | 15.0 ± 2.0* | 5.0 ± 2.9 |
Values are mean ± SD. Type-2 pulmonary granulomas were induced in wild-type (eotaxin +/+) and eotaxin knockout mice (eotaxin −/−). Additional knockout groups were treated with 10 mg of anti-chemokine antibodies or control IgG at the time of bead challenge. On day 4, pulmonary granulomas were isolated, dispersed, and duplicate cytospin preparations were subjected to a 200-cell differential. Five to six mice per group.
*P < 0.05 compared with control IgG group.