Figure 5.

CXCR3 expression and distribution in thyroid tissue specimens from patients affected by GD. A: High CXCR3 expression in the thyroid of the same patient shown in Figures 1 and 2 ▶ ▶ affected by GD (red color). Section was counterstained with Gill’s hematoxylin. B: Absence of CXCR3 signal in an adjacent section immunostained with an isotype control Ab with irrelevant specificity. C: Higher power magnification showing CXCR3 expression in infiltrating inflammatory cells (red color) in the thyroid of the same patient affected by GD. Section was counterstained with Gill’s hematoxylin. D: Higher power magnification showing CXCR3 expression in endothelial cells (red color) in the thyroid of the same patient affected by GD. Section was counterstained with Gill’s hematoxylin. E: Double immunostaining for CXCR3 (red color) and CD4 (blue color) in the thyroid of the same patient affected by GD demonstrating CXCR3 expression by infiltrating T cells of the CD4 phenotype. No counterstain was applied. F: Double immunostaining for CXCR3 (red color) and CD8 (blue color) in the thyroid of the same patient affected by GD demonstrating CXCR3 expression by infiltrating T cells of the CD8 phenotype. No counterstain was applied. G: Double immunostaining for CXCR3 (red color) and CD68 (blue color) in the thyroid of the same patient affected by GD demonstrating CXCR3 expression by infiltrating monocytes/macrophages. No counterstain was applied. H: Double immunostaining for CXCR3 (red color) and CD11c (blue color) in the thyroid of the same patient affected by GD demonstrating CXCR3 expression by infiltrating dendritic cells. No counterstain was applied. I: Double immunostaining for CXCR3 (red color) and CD83 (blue color) in the thyroid of the same patient affected by GD demonstrating CXCR3 expression by infiltrating dendritic cells. No counterstain was applied. Original magnifications: ×100 (A–B); ×400 (C–I).