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Expression of the proliferation marker Ki-67 in podocytes after a 24-hour incubation. In cultures of untreated podocytes (top) the average number of Ki-67-positive cell cycle-engaged cells was 15 ± 7% of the cells incubated in DMEM-0.1% BSA. After treatment with 10 ng/ml of Tat (bottom), 90 ± 5% of the cultured podocytes expressed the Ki-67 nuclear antigen. Original magnification, ×400.
