Figure 2.

Binding of the gp120/sCD4 complex to sulfated and phosphorylated peptides. The association of different gp120/sCD4 complexes with immobilized biotinylated peptide bP-10/14 (dotted black lines) or bS-10/14 (solid black lines) was analyzed by Biacore. RU values as a function of time were also measured in the absence of peptide (dotted gray lines). Binding studies were performed with the following proteins (100 nM): (a) gp120_JR-FL/sCD4, (b) gp120_JR-FL, (c) sCD4, (d) gp120-ΔV3_JR-FL/sCD4, (e) gp120_DH123/sCD4, (f) gp120_DH123, (g) gp120_LAI/sCD4 and (h) gp120_LAI. A 10-fold dilution (10 nM) of gp120_JR-FL/sCD4 was also tested (solid gray line in a). Representative examples of RU measurements are shown.