Figure 3.
Effect of peptides on mAb binding to CCR5. L1.2-CCR5+ cells were incubated with the anti-CCR5 mAbs in the presence of peptides. The extent of mAb binding in the absence of peptide was defined as 100% (m.f.i. ≈50–400, depending on the mAb) and is represented by a horizontal dotted line. Binding in the presence of peptide is expressed as a percentage of control. When CCR5-negative cells were used, binding of mAbs was negligible (m.f.i. ≈2 ± 1). Each data point represents the mean ± SD of three replicates.