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Binding of mAbs to sulfated and phosphorylated peptides. The association of anti-CCR5 mAbs with immobilized biotinylated peptide bP-10/14 (dotted black lines) or bS-10/14 (solid black lines) was analyzed by Biacore. RU values as a function of time were also measured in the absence of peptide (dotted gray lines). Binding studies were performed with (a) PA8, (b) PA10, and (c) 2D7. Representative examples of RU measurements are shown.
