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. 2002 Jul;161(1):301–311. doi: 10.1016/s0002-9440(10)64182-x

Figure 4.

Figure 4.

Preadsorption of EBA serum against proteins from insect cells infected with recombinant BV-0, lacking NC1-cDNA, does not abolish its blister-inducing capacity. EBA serum was purified against proteins from insect cells infected with recombinant BV-0 coupled to an agarose matrix. A: By indirect IF microscopy, IgG eluted from the column that completely lost indirect IF reactivity (a), in contrast IgG from the flow-through fraction stained the dermal side of salt-split skin (b). B: By immunoblotting, like the original serum (lane 1), the flow-through fraction (lane 2), reacted with recombinant NC1, whereas the eluted fraction (lane 3), like normal human serum (lane 4), was unreactive. C: When incubated with cryosections, in contrast to the eluted fraction (a), the flow-through fraction retained its ability to induce blisters (b). Scale bars, 40 μm.