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The experimental protocol was designed to study whether APB5 affects the vascular system in the kidney glomeruli. The ICR pregnant mice were purchased. The bearing day of the newborn mice was defined as 0 dpp and athe mice were injected intraperitoneally with 100 μg of APB5 or control irrelevant rat IgG daily. Then 1, 3, 9, and 13 dpp mice were killed, and the kidneys were removed.
