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Schematic representation of the hREN/β-Gal transgene. Transgenes were constructed with various lengths of hREN promoter sequences (from 3 to 12 kb) associated with a LacZ reporter gene. The β-galactosidase was targeted to the nucleus with a nuclear localization sequence (NLS), and the matrix attachment region (MAR) was designed to insulate the transgene from insertion positional effects.
