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. 2002 Aug;161(2):481–490. doi: 10.1016/s0002-9440(10)64204-6

Figure 3.

Figure 3.

Localization of GGL by immunohistochemistry and immunofluorescence. A: Section of GGL-deficient spleen. B: Section of WT spleen. The black arrow points to positive staining on endothelium of a sinusoid. C: Section of WT liver. The black arrow points to positive staining on endothelium of a sinusoid; the white arrow points to lack of staining on endothelium of a central vein. D: Section of WT kidney. The black arrow points to positive staining on endothelium of capillaries in a glomerulus; the white arrow points to lack of staining on endothelium of a small artery. E: Section of WT brain. The black arrow points to positive staining on endothelium of a capillary. F: Section of WT heart. The black arrow points to positive staining on endothelium of a capillary in myocardium. G to L: Results of immunofluorescence co-localization of GGL and CD31. G to I: Sections of WT liver. J to L: Sections of WT brain. G and J: GGL expression detected with Oregon Green. H and K: CD31 expression detected with Texas Red. I and L: GGL and CD31 co-localization with Oregon Green and Texas Red merged. Original magnifications, ×400.