Figure 6.

Response of GGL to asthma induction. A: Western blot analysis of GGL protein in lungs from mice treated with PBS or CF. The figure shows the light chain of GGL protein with a molecular weight of 20 kd (see Materials and Methods). Lane 1 is from the lung of a PBS-treated GGL-deficient mouse, used as a negative control for GGL; lanes 2 and 3, samples from PBS-treated WT mice; lanes 4 and 5, samples from CF-treated WT mice; lanes 6 and 7, samples from PBS-treated GGT-deficient mice; and lanes 8 and 9, samples from CF-treated GGT-deficient mice. B: LTC₄/LTD₄ conversion activity in lungs. WT, GGT^−/−, and GGL^−/− mice were treated with either PBS (□) or CF (▪). Conversion of LTC₄ to LTD₄ and to LTE₄ was analyzed by HPLC as described in Materials and Methods. The specific activity is expressed as nmol/mg protein/hour (n = 3 to 6). Values are mean ± SEM. For GGT (assayed in GGL-deficient mice) the difference between PBS treatment and CF treatment, P < 0.05; for GGL (assayed in GGT-deficient mice) the difference between PBS treatment and CF treatment, P < 0.001; both tested by Student’s t-test.