Parasite burden in maternal tissues in late gestation. T. cruzi-infected mice were killed at G17 (open bars) or G19 (filled bars), and intracellular parasites were counted in tissue sections. The state of fetuses from which placentas were obtained is indicated as follows: Mac., macerated (pale white fetuses displaying stunted limb buds); Non mac., nonmacerated (reddish-colored fetuses with well-defined limbs); Imm., immobile reddish-colored fetuses; Mob., mobile, alive, reddish-colored fetuses. Results are expressed as mean ± SEM of n samples per group. Asterisks indicate significant differences between the indicated groups (P < 0.05, Student’s t-test). ND, not determined.