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. 2002 Nov;161(5):1825–1837. doi: 10.1016/s0002-9440(10)64459-8

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Copyright © 2002, American Society for Investigative Pathology

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Figure 4. — Comparison of the results achieved using microarray analysis and quantitative PCR between the two variants. To validate the microarray result, quantitative PCR was performed for eight genes using TaqMan probes (five genes; MAL, SAMSN1, BAG3, STAT1, and STAT3) or SYBR green (three genes; TNFSF7, CAV1, and P2Y5). The genes studied included: ▨, genes up-regulated huT78R (MAL, SAMSN1), ▥, genes whose expression was unchanged between the two variants (STAT1, STAT3), and ▧, genes down-regulated in huT78R (BAG3, TNFSF7, CAV1, P2Y5). For all eight genes studied the microarray results were confirmed and in most cases the results obtained from both techniques were extremely similar and no differences observed were significant.