A: Primer and probe sets used for quantitative PCR using Taqman probes |
|
|
|
|
STAT1-F |
XM_010893 |
722 |
5′-GCTGCGGTTCAGTGAGAGCT-3′ |
|
STAT1-R |
|
886 |
5′-GCCATGACTTTGTAATTGCGAAT-3′ |
|
STAT1-P |
|
784 |
5′-AGAACGGAGGCGAACCTGACTTCCA-3′ |
165 bp |
STAT3-F |
NM_003150 |
2311 |
5′-CCTGAAGCTGACCCAGGTAGC-3′ |
|
STAT3-R |
|
2458 |
5′-CACCTTCACCATTATTTCCAAACTG-3′ |
|
STAT3-P |
|
2630 |
5′-TCTGTGTGACACCAACGACCTGCAGC-3′ |
148 bp |
MAL-F |
NM_002371 |
92 |
5′-CCTGCCCAGTGGCTTCTC-3′ |
|
MAL-R |
|
191 |
5′-GGAGGAGGCCACCAGGAT-3′ |
|
MAL-P |
|
126 |
5′-CCCGACTTGCTCTTCATCTTTGAGTTTAT-3′ |
100 bp |
BAG3-F |
XM_055575 |
1969 |
5′-ACAGCAGCAGCGACTTCAAA-3′ |
|
BAG3-R |
|
2072 |
5′-ACACATCGGTTCCGAGTCTGA-3′ |
|
BAG3-P |
|
2017 |
5′-AACCCAGCAGCACCGTAGCCTCTG-3′ |
104 bp |
SAMSN1-F |
XM_012974 |
443 |
5′-AAAGCCAGTGACTCCATGGATAGT-3′ |
|
SAMSN1-R |
|
541 |
5′-TCGAAAGCTGTCCCGGTT-3′ |
|
SAMSN1-P |
|
475 |
5′-CTTGTTATGCCACTTGATGAGCTCTGTCCA-3′ |
99 bp |
B: Primer pairs used for gene expression studies using SYBR green |
|
|
|
|
CAV1-F |
NM_001753 |
431 |
5′-GGTCAACCGCGACCCTAA-3′ |
|
CAV1-R |
|
512 |
5′-TGTCCCTTCTGGTTCTGCAA-3′ |
82 bp |
TNFSF7-F |
NM_001252 |
293 |
5′-TGCCGCTCGAGTCACTTG-3′ |
|
TNFSF7-R |
|
418 |
5′-CTGGTCCATGCAGGAAGGA-3′ |
126 bp |
P2Y5-F |
AF000546 |
401 |
5′-CTACCCATTTAAGTCAAAGACTCTAAGAACC-3′ |
|
P2Y5-R |
|
548 |
5′-GCAGGCTTCTGAGGCATTGTT-3′ |
148 bp |