Table 4.
Comparison of Serum Phosphate, Calcium, and Alkaline Phosphatase in Wild-Type (+/ + and +/Y), Hemizygous (Ska1/Y), Heterozygous (Ska1/ +), and Homozygous (Ska1/Ska1) Mice
| Sex | Genotype | Number | Calcium (mmol/L) mean ± SD | Phosphorous (mmol/L) mean ± sd | Alkaline phosphatase (IU/l) mean ± SD |
|---|---|---|---|---|---|
| Male | +/Y | n = 19 | 2.47 ± 0.09 | 2.33 ± 0.21 | 156 ± 23 |
| Male | Ska1/Y | n = 14 | 2.41 ± 0.11 | 1.41 ± 0.18* | 494 ± 221* |
| Female | +/ + | n = 18 | 2.47 ± 0.05 | 2.47 ± 0.05 | 192 ± 15 |
| Female | Ska1/ + | n = 7 | 2.34 ± 0.09 | 1.15 ± 0.22* | 338 ± 61* |
| Female | Ska1/Ska1 | n = 15 | 2.27 ± 016* | 1.21 ± 0.17* | 497 ± 121*† |
P values were calculated using the Student’s two-tailed t-test with unequal variance. Comparisons of serum calcium, phosphorous, and alkaline phosphatase levels were made between affected (Ska1/Y) and unaffected (+/Y) males, between affected heterozygous (Ska1/+) and unaffected (+/+) females and between homozygous (Ska1/Ska1) and heterozygous (Ska1/+) females. All testing was adjusted using a Bonferroni correction, which is the most stringent adjustment, by assuming independence of tests, for 12 tests. This reduces the single test significance level from 0.05 to 0.0041. For comparisons to unaffected mice * denotes P < 0.0041 and for comparisons between homozygous and heterozygous female mice † indicates P < 0.0041.