Figure 7.

mRNA expression of TNF-α, IL-1β, IL-6, IFN-γ, and TGF-β1 in the lungs of mutant mice and their wild-type littermates 8 days after intratracheal bleomycin administration. Representative mRNA expression as assessed by RT-PCR amplification of cytokine and β-actin transcripts is shown (A). mRNA expression in wild-type littermates that received intratracheal saline injection served as controls. These results represent those obtained with 5 mice of each genotype. The amount of each cytokine PCR product was quantified and normalized to the level of β-actin (B). All values represent the means ± SEM of results obtained from 5 mice in each group.