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Detection of CD97 isoform mRNA by reverse transcriptase-PCR. A: The primer pair PhCD97EGFs1/r1 overspans the alternatively spliced EGF-like domain-coding region resulting in a 452-bp (EGF 1,2,5), 584-bp (EGF 1,2,3,5), or 731-bp (EGF 1,2,3,4,5) PCR product. B: Amplification of a part of the transmembrane-coding region of CD97 results in a 356-bp product. Positive control, peripheral blood lymphocytes; muscle, muscular coat of the colon; Mr, 100-bp DNA ladder.
