Figure 5.

Expression of co-stimulatory CD86 by tissue-infiltrating DCs in GCA. Frozen sections from temporal artery specimens were stained with Abs against CD86 (A) and CD83 (B). A subset of CD83⁺ DCs expressed CD86 (yellow arrows; CD83⁺CD86⁻, white arrows). To determine the frequency of CD86-expressing DCs, frozen sections were either double-labeled with anti-CD83 and anti-CD86 Abs or serial sections were individually stained with the two Abs. Results of both experimental approaches were identical. Frequencies of CD86⁺CD83⁺ DCs were determined. Results from six patients are shown (C). Original magnifications, ×200 (A and B).