Figure 6.
Tissue production of CCL18, CCL19, and CCL21 in temporal arteries affected by GCA. cDNA was prepared from tissue extracts of temporal artery specimens. Transcripts for β-actin, CCL18, CCL19, and CCL21 were determined using quantitative PCR. Concentrations of chemokine transcripts were adjusted to 2 × 105 β-actin copies and are shown as box plots (median and 25th and 75th percentiles) with whiskers marking the 10th and 90th percentiles. Transcripts of all three chemokines were abundant in arteries with vasculitis. Control arteries were negative for CCL18 and CCL19 but contained low numbers of CCL21 mRNA.