Table 1.
Antibodies Used for Immunohistochemistry
Specificity | Species | Dilution | Vendor |
---|---|---|---|
S-100 | Rabbit anti-cow | 1:1600 | DAKO, Carpinteria, CA |
CD83 | Mouse anti-human | 1:1000 | Research Diagnostics, Flanders, NJ |
CD86 | Goat anti-human | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA |
CD68 | Mouse anti-human | 1:100 | DAKO |
CCL19 (MIP-3β) | Mouse anti-human | 1:10,000 | R&D Systems, Minneapolis, MN |
CCL21 (6cKine) | Goat anti-human | 1:5000 | R&D Systems |
CCR7 | Mouse anti-human | 1:1000 | BD-Pharmingen, San Diego, CA |
CD1a | Mouse anti-human | 1:100 | DAKO |
CD3 | Mouse anti-human | 1:100 | DAKO |
Rabbit Ig | Goat anti-Rabbit | 1:300 | DAKO |
Mouse Ig | Rabbit anti-mouse | 1:300 | DAKO |
Goat Ig | Rabbit anti-goat | 1 :300 | DAKO |