Table 1.

Histological Severity of Antigen-, SP-, and LPS-Induced Cystitis

Stimulus	Edema	PMNs	Mast cells*
Saline	0.10 ± 0.14	0.18 ± 0.14	8.0 ± 0.7
LPS	2.70 ± 0.10†	2.30 ± 0.10†	10.1 ± 1.7
SP	2.10 ± 0.20†	2.20 ± 0.20†	8.4 ± 2.6
Antigen	2.88 ± 0.06†	2.42 ± 0.11†	7.5 ± 0.4‡

The histologic severity of cystitis was graded by a score of 0–3.

*Number of mast cells per bladder cross-section. Bladder tissue was fixed in formalin and then paraffin-embedded. Four 5-μm serial sections (8 μm apart) were stained with hematoxylin and eosin for histological analysis. Additional sections were stained with Giemsa for evaluation of mast cell numbers. Values are means ± SEM. P values were obtained using Wilcoxon’s rank sum test.

^†P < 0.0001 vs. the respective control (instilled with saline).

^‡Not significant when compared with the respective control (instilled with saline).