Table 3.
Histopathology Scores for IL-10 KO Mice Colonized with E. faecalis
| Group | No. of mice | Tissue affected | Histopathology scores* | Total | |||
|---|---|---|---|---|---|---|---|
| I | II | III | IV | ||||
| I Early† 5 to 15 weeks after colonization | 19‡ | Cecum | 2/19 | 1/19 | 0/19 | 0/19 | 3/19 |
| Colon | 1/19 | 3/19 | 7/19 | 0/19 | 11/19 | ||
| Rectum | 2/19 | 1/19 | 10/19 | 3/19 | 16/19 | ||
| II Late 20 to 28 weeks after colonization | 16 | Cecum | 10/16 | 2/16 | 0/16 | 3/16 | 15/16 |
| Colon | 1/16 | 0/16 | 11/16 | 3/16 | 15/16 | ||
| Rectum | 0/16 | 1/16 | 8/16 | 7/16 | 16/16 | ||
| Late colonized from birth to 25 weeks | 5 | Cecum | 0/5 | 1/5 | 0/5 | 0/5 | 1/5 |
| Colon | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | ||
| Rectum | 0/5 | 0/5 | 2/5 | 3/5 | 5/5 | ||
*Grade I, Thickness of intestine three times normal with hyperplastic glands, a reduction in mucus-secreting goblet cells, pseudostratification of glandular lining epithelium, or stratification (two to three cells in thickness). Increased mucosal lymphoid cells including plasma cells with or without infiltration through the muscularis mucosae. Mucosal lymphoid tissue was excluded. Grade II, The above features (i.e., grade I) and scattered crypt abscesses—usually five or more PMN/crypt. The mucosal infiltrate included a progressive increase in the number of PMN from grade II to grade IV. Grade III, Features of grade I and multiple crypt abscesses, at least three per ×10 field. Grade IV, The above features or grade I, II, or III with ulceration often accompanied by glandular distortion.
†Indicates length of time mice were colonized by E. faecalis. Mice were colonized at 7 to 17 weeks of age, except those colonized at birth.
‡Number of mice colonized with E. faecalis.