Figure 1.

Pro-MT1-MMP processing. A: Schematic representation of the structural aspects of the human pro-MT1-MMP (63 kd) and its PC-processing sites at the RRPR⁹²↓ and RRKR¹¹¹↓ The mature enzyme (63 kd) can be further autocatalytically cleaved (itrio) into a 45-kd C-terminal form. Shown are the pro-, catalytic (with the HExxH signature), and hemopexin-like domains. B: Western blots of the cell lysates obtained from the colon carcinoma cell line HT-29 transfected with pIRES2-EGFP vector alone (HT-29) or a recombinant vector containing cDNA of α1-PDX (HT-29/PDX) using mouse anti-human MT1-MMP monoclonal antibody (mAb 3319) recognizing the catalytic domain.