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Total cellular RNA was examined by Northern blotting for the expression of α-SMA, collagen-I, MMP 2, TIMP-1, and TIMP-2. A single signal of appropriate size was observed for each of the mRNAs for α-SMA, procollagen-1, MMP 2, and TIMP-1 whereas TIMP-2 mRNA was expressed as two splice variants of 1.0 and 3.4 kb from activated PSCs passaged two to four times. Similar results were observed in four separate preparations of PSCs.
