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Microvessels were isolated from the brains of patients with AD (n = 10) or nondemented elderly controls (n = 5). Brain microvessels were washed and incubated for 6 hours with serum-free DMEM containing 1% lactalbumin hydrogenase, centrifuged, and the supernatant assayed for TGF-β using an ELISA-based assay. *, P < 0.05, significantly different from control-derived microvessels.
