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Microvessels were isolated from the brains of patients with AD or nondemented elderly controls. Microvessel lysates (50 μg/lane) were loaded for SDS-polyacrylamide gel electrophoresis, and Western blot analyses and were performed using antibodies to TGF-β. Lanes 1–3, non-AD control-derived microvessels; lanes 4–6, AD-derived vessels.
