Figure 4.

Confluent endothelial cells were incubated in serum-free DMEM with 0.1% bovine serum albumin and then exposed to different concentrations of TGF-β for 24 hours (lanes 2–4 and 6–8). Control (unstimulated) cells were incubated in media alone (lanes 1 and 5). Endothelial cells were washed, scraped, and samples prepared for SDS-polyacrylamide gel electrophoresis. Western blot analysis was performed using antibodies to IL-1β (left) and TNF-α (right).