Figure 3.

Immunohistochemistry for E-cadherin (a and b) and β-catenin (c and d). Confluent monolayers of HK-2 cells were stimulated with TGF-β1 for 4 days before fixation and analysis by immunohistochemistry as described in Materials and Methods. Under control conditions (serum-free medium alone) both E-cadherin (a) and β-catenin (c) clearly outline the cell contour. After stimulation with recombinant TGF-β1 E-cadherin staining intensity becomes weaker and discontinuous (b). β-catenin staining after addition TGF-β1 demonstrates a relocation from the cell periphery to a perinuclear location (d).