Table 1.
Northern Blot Analysis of 8q24 Genes/ESTs in Normal Prostate Tissue, PC3, and DU145 Cell Lines*
| NCBI accession no. of EST/known gene | T/N Ratio† | PC3/DU145 | ||
|---|---|---|---|---|
| PC3 | DU145 | |||
| Group I (21 genes/ESTs): not detected in either PC3 or DU145 | AA195113/TNFRSF11B | –‡ | – | – |
| AI033054/SNTB1 | – | – | – | |
| AA041285/COL14A1 | – | – | – | |
| AA115496/PFDN2 | – | – | – | |
| AI027678/KIAA0429§ | – | – | – | |
| N35082 | – | – | – | |
| AI373141 | – | – | – | |
| AA652226 | – | – | – | |
| AA131866 | – | – | – | |
| AA662447 | – | – | – | |
| H99761 | – | – | – | |
| H43165 | – | – | – | |
| R06440 | – | – | – | |
| T83344 | – | – | – | |
| AA234000 | – | – | – | |
| N54807 | – | – | – | |
| AA134929 | – | – | – | |
| H47317 | – | – | – | |
| AA236935 | – | – | – | |
| H81291 | – | – | – | |
| AI093325 | – | – | – | |
| Group II (6 genes/ESTs): not overexpressed either in PC3 or DU145 compared to normal prostate | N33223 | 2.1 ± 0.1 | 1.2 ± 0.2 | 1.8 ± 0.2 |
| AA505468 | 1.7 ± 0.2 | 0.7 ± 0.1 | 2.4 ± 0.5 | |
| AI014960 | 1.7 ± 0.0 | 0.8 ± 0.1 | 2.0 ± 0.3 | |
| AA012865 | 1.7 ± 0.2 | 0.7 ± 0.0 | 2.4 ± 0.4 | |
| AA039790 | 0.8 ± 0.2 | 0.9 ± 0.1 | 0.9 ± 0.3 | |
| AI139599/PSCA¶ | 0.08 ± 0.01 | 0.02 ± 0.00 | 4.5 ± 0.9 | |
| Group III (5 genes/ESTs): overexpressed both in PC3 and DU145 compared to normal prostate | BG503734/RAD21 | 4.3 ± 1.7 | 2.6 ± 0.3 | 1.6 ± 0.6 |
| AA253165/EXT1 | 3.5 ± 2.5 | 2.2 ± 1.0 | 1.5 ± 0.4 | |
| AA400196/SQLE | 5.3 ± 1.8 | 5.5 ± 0.1 | 1.0 ± 0.3 | |
| AA975567/C-MYC | 5.5 ± 0.4 | 3.2 ± 0.3 | 1.7 ± 0.0 | |
| AI168842/KIAA1249§ | 3.6 ± 0.8 | 2.8 ± 0.9 | 1.8 ± 0.5 | |
| Group IV (7 genes/ESTs): overexpressed greater than 2 fold in PC3 relative to DU145 | AA604355/NOV | 2.4 ± 0.5 | 0.2 ± 0.1 | 15.0 ± 7.0 |
| AA024720/EIF3S3 | 4.2 ± 0.3 | 1.9 ± 0.8 | 2.4 ± 0.6 | |
| AA279171/PRO2577§ | 3.6 ± 0.3 | 1.2 ± 0.0 | 2.9 ± 0.4 | |
| AA031811/KIAA0196§ | 3.1 ± 0.6 | 1.5 ± 0.9 | 2.3 ± 1.0 | |
| AA166821/TRC8 | 2.8 ± 0.1 | 0.8 ± 0.2 | 3.5 ± 0.9 | |
| AI142961/HAS2 | +∥ | − | ∞ | |
| R69368/BM009§ | 11.6 ± 2.0 | 3.7 ± 0.5 | 3.1 ± 0.2 | |
*Data are represented as mean ± SD.
†Tumor/normal ratio adjusted for b-actin expression.
‡−, No detectable expression either in PC3 or DU145.
§Hypothetical proteins.
¶Located in 8q24.3.
∥No detectable expression in normal tissue.