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. 2002 May;160(5):1655–1667. doi: 10.1016/S0002-9440(10)61113-3

Figure 5.

Figure 5.

Characterization of affinity-isolated cortical Lewy bodies from DLB brain. A: Schematic diagram of Percoll density gradient fractions that contained LB-rich material (LB-positive) isolated by magnetic beads coupled to sheep anti-αS antibody. 41 B–D: PAGE and Western blotting (WB) of equal volumes of extracts from normal (LB-negative) and DLB (LB-positive) brain, and lysates (16 μg) of mycParkin-expressing transfected cells (293, CHO). Antibodies depicted are to the N-terminus of parkin (HP6A) and to αS (LB509, syn-1) (B); to parkin’s linker domain (HP1A) and to Ub (C); and to the in-between-RING domain of parkin (HP2A and HP2A abs.) (D). Note, in LB-positive material anti-parkin antibodies detect mature 53-kd parkin, an 11- to 12-kd fragment (open arrowheads), and gel-excluded high Mr parkin isoforms (asterisks). Anti-αS antibodies faintly recognize an ∼22-kd protein (black arrowhead). Brackets indicate oligomeric proteins.