Table 1.
Detection of Cytoplasmic Lewy Bodies (LBs) with Anti-Parkin Antibody in Midbrains from LB Disorders
| A. Conventional immunohistochemistry of serial sections examined by light microscopy | ||||
|---|---|---|---|---|
| Case no. | Clinical diagnosis | LB detected by anti-parkin (hemi-midbrains counted) | LB detected by anti-αS (hemi-midbrains counted) | Ratio anti-parkin to anti-αS |
| 1 | Sporadic PD | 19 (2) | 22 (2) | 0.86 |
| 2 | Sporadic PD | 4 (1) | 12 (2) | 0.67 |
| 3 | DLB | 45 (4) | 43 (4) | 1.05 |
| 4 | DLB | 119 (2) | 208 (2) | 0.57 |
| 5 | αS-linked PD | 4 (4) | 1 (1) | 1.00 |
| 6 | parkin-linked PD | 3 (1) | 2 (1) | 1.50 |
| B. Double immunofluorescence labeling of single sections examined by confocal microscopy | |||||
|---|---|---|---|---|---|
| Case no. | Clinical diagnosis | Hemi-midbrains double labeled | LB detected by anti-parkin | LB detected by anti-αS | Percentage (%) of co-labeled LB |
| 1 | DLB | 2 | 60 | 68 | 88.2 |
| 2 | DLB | 2 | 38 | 39 | 97.4 |
| 3 | DLB | 2 | 7 | 8 | 87.5 |
| 4 | DLB | 2 | 9 | 11 | 81.8 |
| 5 | DLB | 2 | 12 | 13 | 92.3 |
Average ratio for sporadic Parkinson’s disease (PD): 0.76; average ratio for dementia with LB (DLB): 0.81.
Average percentage for dementia with LB (DLB): 90.6.