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. 2002 May;160(5):1877–1885. doi: 10.1016/S0002-9440(10)61134-0

Figure 3.

Figure 3.

Higher E2 concentration was required to initiate transcriptional activity of ER in MCs from GS-prone mice. MCs were grown in phenol red-free DMEM/F12 supplemented with 20% charcoal-stripped FBS for 4 days. After transfection, MCs were treated with 0 or 0.1 nmol/L of E2, 10 nmol/L of E2, or 1 μmol/L of ICI for 24 hours in phenol red-free medium containing 10% charcoal stripped FBS. Data are expressed as percentage of vehicle-treated MCs from three independent experiments performed in triplicate.