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. 2002 May;160(5):1877–1885. doi: 10.1016/S0002-9440(10)61134-0

Figure 5.

Figure 5.

E2 up-regulated MMP-9 mRNA expression and activity in MCs from GS-resistant mice, but down-regulated them in MCs from GS-prone mice. MCs were grown for 3 days in phenol red-free DMEM/F12 supplemented with 20% charcoal-stripped FBS. The medium was replaced with phenol red-free DMEM/F12 medium supplemented with 0.1% charcoal-stripped FBS and E2 (0, 0.1, or 1 nmol/L) for 24 hours. A: MMP-9 mRNA expression in the presence of E2 in MCs isolated B6SJL and from ROP mice. B: MMP-9 activity in the presence of E2 in MCs from B6SJL and ROP MCs. Data are expressed as percentage of vehicle-treated MCs (0.001% ethanol, open bars) for each cell type and shown are means ± SEM of three independent experiments. Statistical significance is indicated by *, **, and *** (P < 0.05, P < 0.01, and P < 0.001, respectively) for comparison to vehicle-treated MCs.