Table 1.
Primary Antibodies Used for Immunoselection and Immunostaining
| Antibody/clone | Specificity | Working concentration | Specifications | Source |
|---|---|---|---|---|
| hec 7 | hCD31 | 0.4 μg/ml | Mouse IgG2a | W.A. Muller, New York, NY |
| 561 | hCD34 class III | 10 μg/ml | Mouse IgG2a | G. Gaudernack, Oslo, Norway |
| H18/7 | hE-Selectin | 10 μg/ml | Mouse IgG1 | Becton-Dickinson, Bedford, MA |
| NaM185-2C3 | hDARC | 1/10 | Mouse IgG1 | Y. Colin, Paris, France |
| RRI/1.1.1 | hICAM-1 | 10 μg/ml | Mouse IgG1 | R. Rothlein, Ridgefield, CT |
| CBR-C2/2 | hICAM-2 | 1 μg/ml | Mouse IgG2a | Bender Med Systems, Vienna, Austria |
| 4B9 | hVCAM-1 | 3.5 μg/ml | Mouse IgG1 | J. Harlan, Seattle, WA |
| OKM5 | hCD 36 | 1/200 | Mouse IgG1 | Ortho Systems Inc., Raritan, NJ |
| 4.8.G | hVE-cadherin | 1/1000 | Mouse IgG1 | O. Bakke, Oslo |
| IV.3 | hCD 32 | 1/100 | Mouse IgG1 | L. Bjoerge, Bergen, Norway |
| B8.11 | HLA-DR | 1.3 μg/ml | Mouse IgG2b | B. Malissen, Marseille, France |
| MEC 13.3 | mCD31 | 1/100 | Rat IgG2a | A. Vecchi, Milano, Italy |
| YN 1.1.7.4 | mICAM-1 | 10 μg/ml | Rat Ig | F. Takei, Vancouver, Canada |
| MK 27 | mVCAM-1 | 10 μg/ml | Rat Ig | P. Kincade, Oklahoma City, OK |
| von Willebrand factor | vWf | 4.1 μg/ml | Rabbit IgG | DAKO, Glostrup, Denmark |
| 42/2 | Potato virus | 10 μg/ml | Mouse IgG2a | R. Burns, Edinburgh UK |
| 60/3.4 | Tomato nepovirus | Corresponding to relevant Ab | Mouse IgG1 | R. Burns |
| F4/80 | Monocytes/ macrophages | 10 μg/ml | Rat IgG2b | S. Gordon, Oxford, UK |
| MIB 1 | Ki67 | 6.7 μg/ml | Mouse IgG1 | Dianova, Hamburg, Germany |
| M 0851 | Smooth muscle actin | 10 μg/ml | Mouse IgG2a | DAKO |