Figure 2.

Identification of HDC in SCLC cell lines and SCLC tumors by immunoblotting and RT-PCR. A Western blot of protein extracts of the SCLC cell lines H24 and H69 and a snap-frozen tumor (TU, case 13 in Table 2 of reference 2 is shown in A). We observed two bands with the expected molecular weight indicating the presence of both functional forms of HDC. The molecular weight of the bands is indicated on the left. We performed nested RT-PCR with mRNA extracted from the SCLC cell lines H24 and H69 (B) and of paraffin sections of resected SCLC tumors (C) (P1 and P2, cases 2 and 12 in Table 2 of reference 2 ) and of a biopsy (P3, case 9 in Table 1 ▶ ). We obtained a band of the expected size indicating the presence of RNA coding for HDC. PCR reaction without input cDNA served as negative controls (Co) and stomach (S) as a positive control. The identity of the PCR products was confirmed by sequencing.