Figure 6.

EP₂AS clones showed significantly greater depth of invasion compared with empty vector (Mirb) or EP₂ sense clones. Depth of invasion (in μm) was quantitated in H&E-stained sections of formalin-fixed, paraffin-embedded skin equivalents by measuring the five deepest points of invasion for each clone. Values represent the mean and SD from two to four separate skin equivalents for each clone. Values equal 123.3 ± 96.9 μm, 308.0 ± 58.1 μm, and 161.1 ± 22.7 μm for Mirb, EP₂AS, and EP₂ sense clones, respectively (***, P < 0.001 compared with vector controls or EP₂ sense clones).