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. 2002 Dec;161(6):2087–2098. doi: 10.1016/S0002-9440(10)64487-2

Figure 1.

Figure 1.

Abnormal ERK distribution in human Lewy body diseases. A–G: Midbrain sections from PD (A), age-matched control (B), or DLB (C, E–G) patients were stained for P-ERK using antibodies from Calbiochem (A–C), Sigma (E, F), and Promega (G). Note abnormal cytoplasmic distribution of P-ERK (coarse red granules of varying sizes) in endogenously pigmented substantia nigra neurons (fine brown uniform granules). D: Nonimmune rabbit serum was used as a negative staining control. H–J: Representative images from PD (H), DLB (I), and control (J) patients stained for T-ERK. Note discrete T-ERK granules in the diseased cases (H and I, arrows) against a background of mottled cytoplasmic staining. K–M: Double-label confocal immunofluorescence study of a PD case showing association (yellow) of P-ERK granules (green) at the periphery of ubiquitinated (red) Lewy bodies. N–P: Double-label confocal immunofluorescence study of a DLB case showing association and partial co-localization (yellow) of P-ERK granules (green) with abnormal α-synuclein aggregates (red). Scale bars, 50 μm.