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. 2002 Dec;161(6):2099–2109. doi: 10.1016/S0002-9440(10)64488-4

Figure 2.

Figure 2.

HoxD10 impairs EC migration. a: Relative levels of HoxD10 mRNA and protein in control and HoxD10-transfected HMEC-1. Top: RT-PCR and the relative amounts of the 499-bp product corresponding to HoxD10 mRNA. The relative RNA loading is shown by the 390-bp band corresponding to 18s ribosomal RNA determined using competitive primers. Bottom: Western blotting of control and HoxD10-transfected HMEC-1 lysates probed with a polyclonal antibody against HoxD10. The arrow indicates the position of the HoxD10 protein. b: Relative migration of control or HoxD10-transfected HMEC-1 on membranes coated with 10 μg of collagen. Migration was assessed after 3 hours in the presence of 50 ng/ml of bFGF (▨) or 50 ng/ml VEGF (▪) or with no treatment (□). Each panel presents the mean of eight individual determinations and error bars show the SD. **, P < 0.05. c: Western blot showing levels of the 125-kd VEGF receptor 2 (top) and total phosphotyrosine content of the immunoprecipitated VEGF receptor 2 (bottom) from control- or HoxD10-transfected HMEC-1 in the presence (+) or absence (−) of 100 ng/ml of VEGF for 30 minutes.