Figure 3.

Disease-associated prion protein (PrP) co-localizes with immunocompetent cells in vessel walls. a–d: PrP co-localization (indicated by an arrow) with HLA-DR in the media of a leptomeningeal artery in sporadic Creutzfeldt-Jakob disease (sCJD) (3F4/HLA-DR double immunofluorescence; magnification, ×600; green color represents PrP, red HLA-DR represents immunopositivity, blue color indicates the nuclei; Int, intima; Med, media of vessel wall). All colors are combined in d. In the lumen of the artery red blood cells and between intima and media, the internal elastic layer shows autofluorescence. e–h: PrP co-localization (indicated by an arrow) with CD68 in the intima of a basal artery in sCJD (3F4/CD68 double immunofluorescence; magnification, ×1000; green color represents PrP, red CD68 indicates immunopositivity, blue color indicates the nuclei; Int, intima; Med, media of vessel wall). All colors are combined in h. i–l: PrP co-localization with S-100 in the intima of a basal artery in sCJD (3F4/CD68 double immunofluorescence; magnification, ×600; green color represents PrP, red S-100 represents immunopositivity, blue color indicates the nuclei; Int, intima; Med, media of vessel wall). All colors are combined in l. Between intima and media, the internal elastic layer (i and l) shows autofluorescence.