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. Author manuscript; available in PMC: 2007 Apr 10.
Published in final edited form as: Science. 2005 Sep 2;309(5740):1534–1539. doi: 10.1126/science.1113150

Fig. 4.

Fig. 4

(A) The 27-mer R/G site RNA substrate used to assay hADAR2 editing activity. (B) Editing of the R/G site RNA by hADAR2 expressed in wild-type or ipk1Δ yeast. The R/G site adenosine was labeled with 32P and incubated with increasing concentrations of expressed hADAR2 in extracts. Reacted RNA was treated with nuclease P1, the resulting 5′ nucleotide monophosphates separated by thin-layer chromatography (TLC), and the plate exposed to a PhosphorImager screen. The amount of hADAR2 in each extract was determined by Western blotting, and extract was added to give the final ADAR2 concentrations as indicated. (C) Western blot showing the amount of hADAR2 in each reaction. Single-letter abbreviations for amino acid residues are defined in (42).