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. Author manuscript; available in PMC: 2008 Jan 2.
Published in final edited form as: Mol Cell Endocrinol. 2006 Oct 23;260-262:83–92. doi: 10.1016/j.mce.2006.06.012

Figure 1. Interactions of knobbed analogs with FSHR2 revealed the presence of cryptic receptor that recognizes hCG in a fashion similar to that of the LHR.

Figure 1

CHO cells that express FSHR2 were incubated with 125I-hFSH (panels A, B, C) or 125I-hCG (panels D, E, F) and increasing amounts of hCG, hFSH, hFSH analog containing knobs at α2 residues 42 or 48 (panels A, D), hCG containing knobs at α2 residues 42 or 48 (panels B, E), or chimera CFC101-109 containing knobs at α2 residues 42 or 48 (panels C, F). The results described in the left panels were obtained in a single experiment. Those described in the right panels were obtained in a single experiment done two days later using the same hormone and analog preparations to make the results comparable. Note also, that the dose response curves illustrated for hCG and hFSH standards in panels A, B, C are the same data; those for hCG and hFSH standards in panels D, E, F are the same data. We copied the hCG and hFSH dose response curves into each panel to make it easier for readers to determine the potencies of the knobbed analogs relative to those of the hormone standards. All values are triplicates and the vertical lines extend to the limits of the SEM.